hSyn-Aion-citrine
(Plasmid
#192569)
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PurposeAAV-vector for optogenetic long-term silencing of neurons
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 192569 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepAAV2
- Backbone size w/o insert (bp) 4363
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Vector typeMammalian Expression, AAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAion
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SpeciesSynthetic; Chlamydomonas reinhardtii
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Insert Size (bp)1659
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MutationT59S, E83N, E90Q, E101S, V117R, E123S, C128A,D156C, T159G, G163A, V242R, T246N, N258Q, E273S
- Promoter human synapsin
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Tag
/ Fusion Protein
- Citrine (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
hSyn-Aion-citrine was a gift from Simon Wiegert (Addgene plasmid # 192569 ; http://n2t.net/addgene:192569 ; RRID:Addgene_192569) -
For your References section:
Aion is a bistable anion-conducting channelrhodopsin that provides temporally extended and reversible neuronal silencing. Rodriguez-Rozada S, Wietek J, Tenedini F, Sauter K, Dhiman N, Hegemann P, Soba P, Wiegert JS. Commun Biol. 2022 Jul 9;5(1):687. doi: 10.1038/s42003-022-03636-x. 10.1038/s42003-022-03636-x PubMed 35810216
