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Addgene

MMTV-GAL4-SV40
(Plasmid #19255)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 19255 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    MMTV-SV40-Bssk
  • Backbone size w/o insert (bp) 7023
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GAL4
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1000

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer pBluescriptKS
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MMTV-GAL4-SV40 was a gift from Philip Leder (Addgene plasmid # 19255 ; http://n2t.net/addgene:19255 ; RRID:Addgene_19255)

  • For your References section:

    Binary system for regulating transgene expression in mice: targeting int-2 gene expression with yeast GAL4/UAS control elements. Ornitz DM, Moreadith RW, Leder P. Proc Natl Acad Sci U S A 1991 Feb 1;88(3):698-702. 10.1073/pnas.88.3.698 PubMed 1846961
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