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Addgene

pENTR4 PARP2 E614Q
(Plasmid #192545)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 192545 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pENTR4
  • Backbone manufacturer
    Thermo Fisher
  • Backbone size w/o insert (bp) 2235
  • Total vector size (bp) 4146
  • Vector type
    Gateway entry vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    poly(ADP-ribose) polymerase 2 E614Q
  • Alt name
    PARP2
  • Alt name
    AT4G02390
  • Species
    A. thaliana (mustard weed)
  • Insert Size (bp)
    1911
  • Mutation
    changed Glu 614 to Gln
  • Entrez Gene
    AT3G18250 (a.k.a. AT3G18250)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer CCCGCCATAAACTGCCAGG
  • 3′ sequencing primer CGTTGAATATGGCTCATAACACCC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pENTR4 PARP2 E614Q was a gift from Lennart Wirthmueller (Addgene plasmid # 192545 ; http://n2t.net/addgene:192545 ; RRID:Addgene_192545)

  • For your References section:

    The superior salinity tolerance of bread wheat cultivar Shanrong No. 3 is unlikely to be caused by elevated Ta-sro1 poly-(ADP-ribose) polymerase activity. Vogt S, Feijs K, Hosch S, De Masi R, Lintermann R, Loll B, Wirthmueller L. Plant Cell. 2022 Aug 18. pii: 6671231. doi: 10.1093/plcell/koac261. 10.1093/plcell/koac261 PubMed 35980152
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