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Addgene

pPIP1;4-NLS-3xmCitrine
(Plasmid #192524)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 192524 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pMpGWB123
  • Backbone manufacturer
    13428
  • Total vector size (bp) 13972
  • Vector type
    Plant Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    PLASMA MEMBRANE INTRINSIC PROTEIN 1;4
  • Alt name
    PIP1;4
  • Species
    A. thaliana (mustard weed)
  • Insert Size (bp)
    2500
  • GenBank ID
    AT4G00430
  • Entrez Gene
    PIP1;4 (a.k.a. AT4G00430, A_IG005I10.2, A_IG005I10_2, F5I10.2, F5I10_2, PIP1E, PLASMA MEMBRANE INTRINSIC PROTEIN 1E, TMP-C, TRANSMEMBRANE PROTEIN C, plasma membrane intrinsic protein 1;4)
  • Promoter PIP1;4
  • Tags / Fusion Proteins
    • NLS (C terminal on insert)
    • 3xmCitrine (C terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPIP1;4-NLS-3xmCitrine was a gift from Gitta Coaker (Addgene plasmid # 192524 ; http://n2t.net/addgene:192524 ; RRID:Addgene_192524)
  • For your References section:

    Single-cell profiling of Arabidopsis leaves to Pseudomonas syringae infection. Zhu J, Lolle S, Tang A, Guel B, Kvitko B, Cole B, Coaker G. Cell Rep. 2023 Jun 20;42(7):112676. doi: 10.1016/j.celrep.2023.112676. 10.1016/j.celrep.2023.112676 PubMed 37342910