pPIP1;4-NLS-3xmCitrine
(Plasmid
#192524)
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PurposeFluorescent reporter for plant responses to bacterial infection
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 192524 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepMpGWB123
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Backbone manufacturer13428
- Total vector size (bp) 13972
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Vector typePlant Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namePLASMA MEMBRANE INTRINSIC PROTEIN 1;4
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Alt namePIP1;4
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SpeciesA. thaliana (mustard weed)
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Insert Size (bp)2500
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GenBank IDAT4G00430
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Entrez GenePIP1;4 (a.k.a. AT4G00430, A_IG005I10.2, A_IG005I10_2, F5I10.2, F5I10_2, PIP1E, PLASMA MEMBRANE INTRINSIC PROTEIN 1E, TMP-C, TRANSMEMBRANE PROTEIN C, plasma membrane intrinsic protein 1;4)
- Promoter PIP1;4
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Tags
/ Fusion Proteins
- NLS (C terminal on insert)
- 3xmCitrine (C terminal on backbone)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer TGTAAAACGACGGCCAGT (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pPIP1;4-NLS-3xmCitrine was a gift from Gitta Coaker (Addgene plasmid # 192524 ; http://n2t.net/addgene:192524 ; RRID:Addgene_192524) -
For your References section:
Single-cell profiling of Arabidopsis leaves to Pseudomonas syringae infection. Zhu J, Lolle S, Tang A, Guel B, Kvitko B, Cole B, Coaker G. Cell Rep. 2023 Jun 20;42(7):112676. doi: 10.1016/j.celrep.2023.112676. 10.1016/j.celrep.2023.112676 PubMed 37342910