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Addgene

TL021_lenti_CMV:SEP-GluA1
(Plasmid #192515)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 192515 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    FCMV
  • Backbone size w/o insert (bp) 8500
  • Total vector size (bp) 12060
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    SEP-GluA1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    3519
  • Promoter CMV
  • Tag / Fusion Protein
    • super ecliptic phluorin (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CMV Forward: CGCAAATGGGCGGTAGGCGTG
  • 3′ sequencing primer WPRE-R: CATAGCGTAAAAGGAGCAACA
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    TL021_lenti_CMV:SEP-GluA1 was a gift from Adam Cohen (Addgene plasmid # 192515 ; http://n2t.net/addgene:192515 ; RRID:Addgene_192515)

  • For your References section:

    Mapping memories: pulse-chase labeling reveals AMPA receptor dynamics during memory formation. Kim D, Park P, Li X, Wong Campos JD, Tian H, Moult EM, Grimm JB, Lavis L, Cohen AE. bioRxiv. 2023 May 29:2023.05.26.541296. doi: 10.1101/2023.05.26.541296. Preprint. 10.1101/2023.05.26.541296 PubMed 37292614
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