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pCMV-PE2-tagRFP-BleoR
(Plasmid #192508)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 192508 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV-PE2 (#132775)
  • Backbone manufacturer
    David Liu
  • Vector type
    Mammalian Expression
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PE2-P2A-tagRFP-P2A-BleoR
  • Alt name
    Prime editor 2-P2A-tagRFP-P2A-BleoR
  • Species
    Synthetic; SpCas9 is from Streptococcus pyogenes; MMLV_RT is from the Moloney murine leukemia virus
  • Insert Size (bp)
    7512
  • Tags / Fusion Proteins
    • SV40 bpNLS (N terminal on insert)
    • SV40 bpNLS (C terminal on insert)
    • tagRFP (C terminal on insert)
    • BleoR (C terminal on insert)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-PE2-tagRFP-BleoR was a gift from Gerald Schwank (Addgene plasmid # 192508 ; http://n2t.net/addgene:192508 ; RRID:Addgene_192508)

  • For your References section:

    Predicting prime editing efficiency and product purity by deep learning. Mathis N, Allam A, Kissling L, Marquart KF, Schmidheini L, Solari C, Balazs Z, Krauthammer M, Schwank G. Nat Biotechnol. 2023 Jan 16. doi: 10.1038/s41587-022-01613-7. 10.1038/s41587-022-01613-7 PubMed 36646933
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