pFastBac FPH
(Plasmid
#1925)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 1925 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepFastBac
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 4775
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Vector typeInsect Expression
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Selectable markersGentamicin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namepolyhomeotic
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Alt namePH
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SpeciesD. melanogaster (fly)
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Insert Size (bp)5200
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Entrez Geneph-d (a.k.a. Dmel_CG3895, CG3895, DROZFP, Dmel\CG3895, EG:BACN25G24.3, PH, PH-d, PHD, Ph, Ph-d, PhD, ph, ph-D, ph[D], ph[d], phd, phm)
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Entrez Geneph-p (a.k.a. Dmel_CG18412, CG18412, CG18414, Dmel\CG18412, EG:87B1.5, PH, PH-p, PHP, Ph, Ph-p, PhP, dPh, ph, ph-P, ph-proximal, ph[P], ph[p], phm, php)
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Tag
/ Fusion Protein
- Flag (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer Polyhedrin forward (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
For baculoviral expression of PH with N-terminal flag epitope. (kingston #1323)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFastBac FPH was a gift from Robert Kingston (Addgene plasmid # 1925 ; http://n2t.net/addgene:1925 ; RRID:Addgene_1925) -
For your References section:
Reconstitution of a functional core polycomb repressive complex. Francis NJ, Saurin AJ, Shao Z, Kingston RE. Mol Cell 2001 Sep;8(3):545-56. 10.1016/S1097-2765(01)00316-1 PubMed 11583617
Map uploaded by the depositor.
![](https://media.addgene.org/data/easy-thumbnails/data/97/39/fa4029d6-af60-11e0-90fe-003048dd6500.jpeg.940x940_q85_autocrop.png)