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Addgene

pSpCas9_T2A_mCherry sg1-sg2
(Plasmid #192479)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 192479 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pX459
  • Backbone manufacturer
    Addgene Plasmid #62988
  • Total vector size (bp) 9723
  • Modifications to backbone
    Replaced Puromycin gene with mCherry fluorescent protein and inserted BFPsg1 & BFPsg2 sgRNA's
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SpCas9
  • Tag / Fusion Protein
    • T2A mCherry (C terminal on insert)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSpCas9_T2A_mCherry sg1-sg2 was a gift from Michael Yaffe (Addgene plasmid # 192479 ; http://n2t.net/addgene:192479 ; RRID:Addgene_192479)

  • For your References section:

    Multi-pathway DNA-repair reporters reveal competition between end-joining, single-strand annealing and homologous recombination at Cas9-induced DNA double-strand breaks. van de Kooij B, Kruswick A, van Attikum H, Yaffe MB. Nat Commun. 2022 Sep 8;13(1):5295. doi: 10.1038/s41467-022-32743-w. 10.1038/s41467-022-32743-w PubMed 36075911
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