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Addgene

pLVX_DSB_Spectrum-V2
(Plasmid #192475)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 192475 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLVX
  • Total vector size (bp) 11006
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    BFP1.2, incompleteGFP
  • Promoter CMV

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLVX_DSB_Spectrum-V2 was a gift from Michael Yaffe (Addgene plasmid # 192475 ; http://n2t.net/addgene:192475 ; RRID:Addgene_192475)

  • For your References section:

    Multi-pathway DNA-repair reporters reveal competition between end-joining, single-strand annealing and homologous recombination at Cas9-induced DNA double-strand breaks. van de Kooij B, Kruswick A, van Attikum H, Yaffe MB. Nat Commun. 2022 Sep 8;13(1):5295. doi: 10.1038/s41467-022-32743-w. 10.1038/s41467-022-32743-w PubMed 36075911
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