pAc5.1/V5-HisA-RvCAHS12-GS-AcGFP1
(Plasmid
#192471)
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PurposeTransient expression of RvCAHS12 (fly codon optimized)-GS_linker-AcGFP1 in Drosophila cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 192471 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAc5.1/V5-HisA
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5358
- Total vector size (bp) 5375
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameCAHS12
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Alt nameRvCAHS12
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SpeciesRamazzottius varieornatus
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Insert Size (bp)1656
- Promoter Ac5
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Tag
/ Fusion Protein
- AcGFP1 (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer AC5 (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAc5.1/V5-HisA-RvCAHS12-GS-AcGFP1 was a gift from Takekazu Kunieda (Addgene plasmid # 192471 ; http://n2t.net/addgene:192471 ; RRID:Addgene_192471) -
For your References section:
Stress-dependent cell stiffening by tardigrade tolerance proteins that reversibly form a filamentous network and gel. Tanaka A, Nakano T, Watanabe K, Masuda K, Honda G, Kamata S, Yasui R, Kozuka-Hata H, Watanabe C, Chinen T, Kitagawa D, Sawai S, Oyama M, Yanagisawa M, Kunieda T. PLoS Biol. 2022 Sep 6;20(9):e3001780. doi: 10.1371/journal.pbio.3001780. eCollection 2022 Sep. 10.1371/journal.pbio.3001780 PubMed 36067153