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pAc5.1/V5-HisA-RvCAHS8-GS-AcGFP1
(Plasmid #192470)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 192470 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAc5.1/V5-HisA
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5358
  • Total vector size (bp) 5375
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    CAHS8
  • Alt name
    RvCAHS8
  • Species
    Ramazzottius varieornatus
  • Insert Size (bp)
    1431
  • Promoter Ac5 promoter
  • Tag / Fusion Protein
    • AcGFP1 (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAc5.1/V5-HisA-RvCAHS8-GS-AcGFP1 was a gift from Takekazu Kunieda (Addgene plasmid # 192470 ; http://n2t.net/addgene:192470 ; RRID:Addgene_192470)

  • For your References section:

    Stress-dependent cell stiffening by tardigrade tolerance proteins that reversibly form a filamentous network and gel. Tanaka A, Nakano T, Watanabe K, Masuda K, Honda G, Kamata S, Yasui R, Kozuka-Hata H, Watanabe C, Chinen T, Kitagawa D, Sawai S, Oyama M, Yanagisawa M, Kunieda T. PLoS Biol. 2022 Sep 6;20(9):e3001780. doi: 10.1371/journal.pbio.3001780. eCollection 2022 Sep. 10.1371/journal.pbio.3001780 PubMed 36067153
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