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betaTrCP mVenus mutant reporter
(Plasmid #192459)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 192459 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLenti
  • Backbone manufacturer
    n/a
  • Backbone size w/o insert (bp) 8765
  • Total vector size (bp) 9971
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    CdC25B
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1206
  • Mutation
    aa198-338 of CDC25B. betaTrCP degron sequence (aa268-273) mutated (DDGVD to ADAFVD)
  • GenBank ID
    NM_021873.4
  • Promoter EF1alpha
  • Tags / Fusion Proteins
    • mVenus (C terminal on insert)
    • SV40 NLS (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer caggtgtcgtgaggatcc
  • 3′ sequencing primer tagagcggccgccctcgagg
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    betaTrCP mVenus mutant reporter was a gift from Steven Cappell (Addgene plasmid # 192459 ; http://n2t.net/addgene:192459 ; RRID:Addgene_192459)

  • For your References section:

    Revealing beta-TrCP activity dynamics in live cells with a genetically encoded biosensor. Paul D, Kales SC, Cornwell JA, Afifi MM, Rai G, Zakharov A, Simeonov A, Cappell SD. Nat Commun. 2022 Oct 26;13(1):6364. doi: 10.1038/s41467-022-33762-3. 10.1038/s41467-022-33762-3 PubMed 36289220
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