BALF4-S758T
(Plasmid
#192457)
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PurposeExpresses BALF5 with S758T mutation
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 192457 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCMV5.1
- Backbone size w/o insert (bp) 3045
- Total vector size (bp) 7681
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameBALF5 (S758T)
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SpeciesEpstein-Barr Virus
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Insert Size (bp)3087
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MutationS758T
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Entrez GeneBALF5 (a.k.a. HHV4_BALF5)
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Tag
/ Fusion Protein
- FLAG (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CMV Fwd (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
BALF4-S758T was a gift from Bill Sugden (Addgene plasmid # 192457 ; http://n2t.net/addgene:192457 ; RRID:Addgene_192457) -
For your References section:
How Epstein-Barr Virus Induces the Reorganization of Cellular Chromatin. Rosemarie Q, Kirschstein E, Sugden B. mBio. 2023 Feb 28;14(1):e0268622. doi: 10.1128/mbio.02686-22. Epub 2023 Jan 10. 10.1128/mbio.02686-22 PubMed 36625581