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Addgene

pEGFP-C1-mScarlet-AMPKα2
(Plasmid #192451)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 192451 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone size w/o insert (bp) 3994
  • Total vector size (bp) 6391
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mScarlet-AMPKalpha2
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    2397
  • Entrez Gene
    Prkaa2 (a.k.a. Ampk, Ampka2)
  • Promoter CMV
  • Tag / Fusion Protein
    • mScarlet (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFP-C1-mScarlet-AMPKα2 was a gift from Jin Zhang (Addgene plasmid # 192451 ; http://n2t.net/addgene:192451 ; RRID:Addgene_192451)

  • For your References section:

    Spatial regulation of AMPK signaling revealed by a sensitive kinase activity reporter. Schmitt DL, Curtis SD, Lyons AC, Zhang JF, Chen M, He CY, Mehta S, Shaw RJ, Zhang J. Nat Commun. 2022 Jul 5;13(1):3856. doi: 10.1038/s41467-022-31190-x. 10.1038/s41467-022-31190-x PubMed 35790710
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