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pcDNA3-mCherry-Rheb(C181S)
(Plasmid #192437)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 192437 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone size w/o insert (bp) 5517
  • Total vector size (bp) 6789
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mCherry-Rheb(C181S)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1272
  • Mutation
    Rheb Cys 181 mutated to Ser; abolishes C-terminal farnesylation.
  • Entrez Gene
    RHEB (a.k.a. RHEB2)
  • Promoter CMV
  • Tags / Fusion Proteins
    • 6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag (N terminal on backbone)
    • mCherry (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-mCherry-Rheb(C181S) was a gift from Jin Zhang (Addgene plasmid # 192437 ; http://n2t.net/addgene:192437 ; RRID:Addgene_192437)

  • For your References section:

    Rheb regulates nuclear mTORC1 activity independent of farnesylation. Zhong Y, Zhou X, Guan KL, Zhang J. Cell Chem Biol. 2022 Jun 16;29(6):1037-1045.e4. doi: 10.1016/j.chembiol.2022.02.006. Epub 2022 Mar 15. 10.1016/j.chembiol.2022.02.006 PubMed 35294906
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