HA-VHL A149T-pBabe-puro
(Plasmid #19242)

• Depositing Lab: William Kaelin
• Publication: Li et al Mol Cell Biol. 2007 Aug . 27(15):5381-92.

Backbone

• Vector backbone: HA-pBabe-puro
• Backbone size w/o insert (bp): 5202
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: von Hippel-Lindau tumor suppressor (VHL)
• Alt name: vHL
• Species: H. sapiens (human)
• Insert Size (bp): 652
• Mutation: changed Alanin 149 to Threonin
• GenBank ID: NM_000551
• Entrez Gene: VHL (a.k.a. HRCA1, RCA1, VHL1, pVHL)
• Tag / Fusion Protein:
  • HA-tag (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamH I (not destroyed)
• 3′ cloning site: EcoR I (not destroyed)
• 5′ sequencing primer: pBabe-5'
• 3′ sequencing primer: pBabe-3'

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry