LLP756_L2pV1_NOT_B3RT_Rluc_1IB_lacZ
(Plasmid
#192401)
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PurposeOff state for the first generation of NOT gates, which target the Rluc CDS to repress expression via its B3RT sites.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 192401 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAGM4673
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Backbone manufacturerSylvestre Marillonnet
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Vector typePlant Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature30°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameAct2::B3RT-Rluc-B3RT
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SpeciesRenilla reniformis
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Tag
/ Fusion Protein
- PEST (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Other genes: TCTP1::Fluc, TCTP1::B3o
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
LLP756_L2pV1_NOT_B3RT_Rluc_1IB_lacZ was a gift from Ryan Lister (Addgene plasmid # 192401 ; http://n2t.net/addgene:192401 ; RRID:Addgene_192401) -
For your References section:
Synthetic memory circuits for stable cell reprogramming in plants. Lloyd JPB, Ly F, Gong P, Pflueger J, Swain T, Pflueger C, Fourie E, Khan MA, Kidd BN, Lister R. Nat Biotechnol. 2022 Jul 4. pii: 10.1038/s41587-022-01383-2. doi: 10.1038/s41587-022-01383-2. 10.1038/s41587-022-01383-2 PubMed 35788565