pCDM8-HEB
(Plasmid
#1924)
-
Depositing Lab
-
Publication
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 1924 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepCDM8
- Backbone size w/o insert (bp) 4350
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin and Tetracycline, 100 & 10 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert nameHEB
-
Alt nameE-protein class of basic helix loop helix
-
SpeciesH. sapiens (human)
-
Insert Size (bp)2450
-
GenBank IDM80627
-
Entrez GeneTCF12 (a.k.a. CRS3, HEB, HH26, HTF4, HsT17266, TCF-12, bHLHb20, p64)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
full length HEB cDNA in CDM8. Vector contains SV40 origin, P(cmv), and SV40 poly A. (kingston #559)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pCDM8-HEB was a gift from Robert Kingston (Addgene plasmid # 1924) -
For your References section:
HEB, a helix-loop-helix protein related to E2A and ITF2 that can modulate the DNA-binding ability of myogenic regulatory factors. Hu JS, Olson EN, Kingston RE. Mol Cell Biol 1992 Mar;12(3):1031-42. PubMed 1312219