LLP736_L2pV1_1I_Act2_FRT_OCS_TCTP_Flp_lacZ
(Plasmid
#192379)
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PurposeTo test if the TCTP promoter can drive the Flp recomhinase in order to remove the OCS terminator and increase circuit output (despite the presence of the lacZ endlinker).
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 192379 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAGM4673
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Backbone manufacturerSylvestre Marillonnet
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Vector typePlant Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature30°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameAct2::FRT-OCS-FRT::Rluc
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SpeciesRenilla reniformis
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Tag
/ Fusion Protein
- PEST (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Other genes: TCTP1::Fluc, TCTP1::FlpO
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
LLP736_L2pV1_1I_Act2_FRT_OCS_TCTP_Flp_lacZ was a gift from Ryan Lister (Addgene plasmid # 192379 ; http://n2t.net/addgene:192379 ; RRID:Addgene_192379) -
For your References section:
Synthetic memory circuits for stable cell reprogramming in plants. Lloyd JPB, Ly F, Gong P, Pflueger J, Swain T, Pflueger C, Fourie E, Khan MA, Kidd BN, Lister R. Nat Biotechnol. 2022 Jul 4. pii: 10.1038/s41587-022-01383-2. doi: 10.1038/s41587-022-01383-2. 10.1038/s41587-022-01383-2 PubMed 35788565