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Addgene

Gly-tRNA-gRNA scaffold for library 2
(Plasmid #192365)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 192365 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Unknown
  • Vector type
    For Golden Gate Assembly

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Gly tRNA-gRNA scaffold
  • Species
    Synthetic

Cloning Information

  • Cloning method Unknown

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Gly-tRNA-gRNA scaffold for library 2 was a gift from Lijia Ma (Addgene plasmid # 192365 ; http://n2t.net/addgene:192365 ; RRID:Addgene_192365)

  • For your References section:

    An in-library ligation strategy and its application in CRISPR/Cas9 screening of high-order gRNA combinations. Lu Z, Ni K, Wang Y, Zhou Y, Li Y, Yan J, Song Q, Liu M, Xu Y, Yu Z, Guo T, Ma L. Nucleic Acids Res. 2022 Jun 24;50(11):6575-6586. doi: 10.1093/nar/gkac458. 10.1093/nar/gkac458 PubMed 35670669
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