Immune checkpoint (library 2) Backbone
(Plasmid
#192363)
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Purpose(Empty Backbone) Plasmid vector for in vivo immune checkpoint screening library (for checkpoint library)
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 192363 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonemodified lentiGuide Puro backbone (Addgene plasmid # 52963) with mKate
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Immune checkpoint (library 2) Backbone was a gift from Lijia Ma (Addgene plasmid # 192363 ; http://n2t.net/addgene:192363 ; RRID:Addgene_192363) -
For your References section:
An in-library ligation strategy and its application in CRISPR/Cas9 screening of high-order gRNA combinations. Lu Z, Ni K, Wang Y, Zhou Y, Li Y, Yan J, Song Q, Liu M, Xu Y, Yu Z, Guo T, Ma L. Nucleic Acids Res. 2022 Jun 24;50(11):6575-6586. doi: 10.1093/nar/gkac458. 10.1093/nar/gkac458 PubMed 35670669
