pBabe NGF-EGFR-FRB-GluGlu neo
(Plasmid
#192293)
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PurposeRetroviral expression vector for NGF-EGFR-FRB-GluGlu construct
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 192293 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBabe neo
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Backbone manufacturerAddgene #1767
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Vector typeRetroviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNGF-EGFR-FRB-GluGlu
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SpeciesH. sapiens (human)
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Entrez GeneEGFR (a.k.a. ERBB, ERBB1, ERRP, HER1, NISBD2, NNCIS, PIG61, mENA)
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Tag
/ Fusion Protein
- GluGlu (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site MfeI (destroyed during cloning)
- 3′ cloning site SalI (not destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBabe NGF-EGFR-FRB-GluGlu neo was a gift from Kevin Janes (Addgene plasmid # 192293 ; http://n2t.net/addgene:192293 ; RRID:Addgene_192293) -
For your References section:
Nucleocytoplasmic transport of active HER2 causes fractional escape from the DCIS-like state. Wang L, Paudel BB, McKnight RA, Janes KA. Nat Commun. 2023 Apr 13;14(1):2110. doi: 10.1038/s41467-023-37914-x. 10.1038/s41467-023-37914-x PubMed 37055441