GST-mCh-FYVE
(Plasmid
#192289)
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PurposeExpresses GST- and mCherry-tagged HRS FYVE in e Coli
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 192289 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGEX-GST
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameHRS FYVE domain
- Promoter tac
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Tag
/ Fusion Protein
- GST-TEV-mCherry (N terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer pGEXforward
- 3′ sequencing primer pGEXreverse (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
GST-mCh-FYVE was a gift from James Hurley (Addgene plasmid # 192289 ; http://n2t.net/addgene:192289 ; RRID:Addgene_192289) -
For your References section:
Membrane curvature sensing and stabilization by the autophagic LC3 lipidation machinery. Jensen LE, Rao S, Schuschnig M, Cada AK, Martens S, Hummer G, Hurley JH. Sci Adv. 2022 Dec 14;8(50):eadd1436. doi: 10.1126/sciadv.add1436. Epub 2022 Dec 14. 10.1126/sciadv.add1436 PubMed 36516251
