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Addgene

GST-mCh-FYVE
(Plasmid #192289)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 192289 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX-GST
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    HRS FYVE domain
  • Promoter tac
  • Tag / Fusion Protein
    • GST-TEV-mCherry (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GST-mCh-FYVE was a gift from James Hurley (Addgene plasmid # 192289 ; http://n2t.net/addgene:192289 ; RRID:Addgene_192289)

  • For your References section:

    Membrane curvature sensing and stabilization by the autophagic LC3 lipidation machinery. Jensen LE, Rao S, Schuschnig M, Cada AK, Martens S, Hummer G, Hurley JH. Sci Adv. 2022 Dec 14;8(50):eadd1436. doi: 10.1126/sciadv.add1436. Epub 2022 Dec 14. 10.1126/sciadv.add1436 PubMed 36516251
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