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pLV-hUbC-GBX2-CDX124-Cas9-T2A-GFP
(Plasmid #192287)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 192287 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLV-hUbC-Cas9-T2A-GFP
  • Total vector size (bp) 15826
  • Vector type
    Mammalian Expression, Lentiviral, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cas9-T2A-GFP and four sgRNA: GBX2 + CDX1 + CDX2 + CDX4
  • Species
    H. sapiens (human), Synthetic
  • Entrez Gene
    GBX2
  • Entrez Gene
    CDX2 (a.k.a. CDX-3, CDX2/AS, CDX3)
  • Entrez Gene
    CDX1
  • Entrez Gene
    CDX4

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

CDX1 gRNA sequence: CTACACCGACCACCAACGCC
CDX2 gRNA sequence: GTACACGGACCACCAGCGGC
CDX4 gRNA sequence: CCTGGGCCTTTCCGAGAGAC
GBX2 gRNA sequence: AAAGGTGGAAGACGACCCGA

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLV-hUbC-GBX2-CDX124-Cas9-T2A-GFP was a gift from Mark Denham (Addgene plasmid # 192287 ; http://n2t.net/addgene:192287 ; RRID:Addgene_192287)

  • For your References section:

    Enhanced production of mesencephalic dopaminergic neurons from lineage-restricted human undifferentiated stem cells. Maimaitili M, Chen M, Febbraro F, Ucuncu E, Kelly R, Niclis JC, Christiansen JR, Mermet-Joret N, Niculescu D, Lauritsen J, Iannielli A, Klaestrup IH, Jensen UB, Qvist P, Nabavi S, Broccoli V, Nykjaer A, Romero-Ramos M, Denham M. Nat Commun. 2023 Dec 5;14(1):7871. doi: 10.1038/s41467-023-43471-0. 10.1038/s41467-023-43471-0 PubMed 38052784
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