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Addgene

pXD71Cas10RFP-Pct5.1-crRNA(AarI)-RFP
(Plasmid #192273)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 192273 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pXD69m2
  • Vector type
    Bacterial Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Kanamycin, 100 & 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CymR
  • Species
    Pseudomonas putida F1
  • GenBank ID
    AAB62296.1

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pXD71Cas10RFP-Pct5.1-crRNA(AarI)-RFP was a gift from Emily Balskus (Addgene plasmid # 192273 ; http://n2t.net/addgene:192273 ; RRID:Addgene_192273)

  • For your References section:

    Genetic manipulation of the human gut bacterium Eggerthella lenta reveals a widespread family of transcriptional regulators. Dong X, Guthrie BGH, Alexander M, Noecker C, Ramirez L, Glasser NR, Turnbaugh PJ, Balskus EP. Nat Commun. 2022 Dec 9;13(1):7624. doi: 10.1038/s41467-022-33576-3. 10.1038/s41467-022-33576-3 PubMed 36494336
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