pCMV-PARP1_D770A-mCherry
(Plasmid #192260)

• Purpose: Expresses PARP1 D770A mutant in mammalian cells. Tagged with mCherry.
• Depositing Lab: Michael Cohen
• Publication: Arnold et al Cell Chem Biol. 2022 Dec 15;29(12):1694-1708.e10. doi: 10.1016/j.chembiol.2022.11.006. Epub 2022 Dec 8.

Backbone

• Vector backbone: pCMV-PARP1-mCherry
• Backbone size w/o insert (bp): 7307
• Total vector size (bp): 7711
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: PARP1_D770A
• Species: H. sapiens (human)
• Insert Size (bp): 404
• Mutation: D770A
• Entrez Gene: PARP1 (a.k.a. ADPRT, ADPRT 1, ADPRT1, ARTD1, PARP, PARP-1, PARS, PPOL, Poly-PARP, pADPRT-1)
• Promoter: CMV
• Tag / Fusion Protein:
  • mCherry (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BlpI (not destroyed)
• 3′ cloning site: EcoRV (not destroyed)
• 5′ sequencing primer: CMV
• 3′ sequencing primer: SV40

Resource Information

• Supplemental Documents:
  • pCMV-PARP1_D770A-mCherry.gbk
• A portion of this plasmid was derived from a plasmid made by: Backbone gifted from Dr. G. Timinszky (Hungarian Academy of Sciences)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry