Skip to main content
Addgene

GFP-GGA3-GAT
(Plasmid #192259)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 192259 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    eGFP-C2
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Total vector size (bp) 5235
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    GGA3-GAT
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    503
  • Entrez Gene
    GGA3
  • Promoter CMV
  • Tag / Fusion Protein
    • GFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer EGFP-C-FOR
  • 3′ sequencing primer SV40pA-R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GFP-GGA3-GAT was a gift from Juan Bonifacino (Addgene plasmid # 192259 ; http://n2t.net/addgene:192259 ; RRID:Addgene_192259)

  • For your References section:

    The GGAs promote ARF-dependent recruitment of clathrin to the TGN. Puertollano R, Randazzo PA, Presley JF, Hartnell LM, Bonifacino JS. Cell. 2001 Apr 6;105(1):93-102. doi: 10.1016/s0092-8674(01)00299-9. 10.1016/s0092-8674(01)00299-9 PubMed 11301005
Related items:
Commonly requested with: