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eIF3a- pMK289 (mAID-mClover-NeoR) plasmid
(Plasmid #192235)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 192235 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCR-Blunt II-TOPO vector(Cat#450245)
  • Backbone manufacturer
    Thermo fisher
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    eIF3a-knockin-homology arm-mAID-mClover-NeoR
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    5058
  • Entrez Gene
    EIF3A (a.k.a. EIF3, EIF3S10, P167, TIF32, eIF3-p170, eIF3-theta, p180, p185)

Cloning Information

  • Cloning method TOPO Cloning
  • 5′ sequencing primer CAGGAAACAGCTATGAC
  • 3′ sequencing primer TGTAAAACGACGGCCAGT
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    eIF3a- pMK289 (mAID-mClover-NeoR) plasmid was a gift from Dieter Wolf (Addgene plasmid # 192235 ; http://n2t.net/addgene:192235 ; RRID:Addgene_192235)

  • For your References section:

    eIF3 mRNA selectivity profiling reveals eIF3k as a cancer-relevant regulator of ribosome content. Duan H, Zhang S, Zarai Y, Ollinger R, Wu Y, Sun L, Hu C, He Y, Tian G, Rad R, Kong X, Cheng Y, Tuller T, Wolf DA. EMBO J. 2023 Jun 15;42(12):e112362. doi: 10.15252/embj.2022112362. Epub 2023 May 8. 10.15252/embj.2022112362 PubMed 37155573
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