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Addgene

pCMV-HA Set2
(Plasmid #192220)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 192220 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV-HA-N
  • Backbone manufacturer
    TAKARA
  • Backbone size w/o insert (bp) 3782
  • Total vector size (bp) 4544
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    not specialized
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Set isoform 2
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    834
  • GenBank ID
    NM001204875
  • Entrez Gene
    Set (a.k.a. 2610030F17Rik, 5730420M11Rik, I-2PP2A, StF-IT-1, TAF-I)
  • Promoter CMV
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer TCTGCGCCGACGGCCAAAGCCAG
  • 3′ sequencing primer CTAATCATCCTCGCCTTCGTCCTCCT
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-HA Set2 was a gift from Hisakazu Ogita (Addgene plasmid # 192220 ; http://n2t.net/addgene:192220 ; RRID:Addgene_192220)

  • For your References section:

    Vascular smooth muscle RhoA counteracts abdominal aortic aneurysm formation by modulating MAP4K4 activity. Molla MR, Shimizu A, Komeno M, Rahman NIA, Soh JEC, Nguyen LKC, Khan MR, Tesega WW, Chen S, Pang X, Tanaka-Okamoto M, Takashima N, Sato A, Suzuki T, Ogita H. Commun Biol. 2022 Oct 7;5(1):1071. doi: 10.1038/s42003-022-04042-z. 10.1038/s42003-022-04042-z PubMed 36207400
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