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Addgene

pWRS3001_VCR1_WCR3
(Plasmid #192206)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 192206 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTWIST
  • Backbone manufacturer
    Twist Bioscience
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    VCR1-WCR3 tracrRNA
  • Species
    Synthetic
  • Insert Size (bp)
    223

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pWRS3001_VCR1_WCR3 was a gift from William Sellers (Addgene plasmid # 192206 ; http://n2t.net/addgene:192206 ; RRID:Addgene_192206)

  • For your References section:

    Comparative optimization of combinatorial CRISPR screens. Li R, Klingbeil O, Monducci D, Young MJ, Rodriguez DJ, Bayyat Z, Dempster JM, Kesar D, Yang X, Zamanighomi M, Vakoc CR, Ito T, Sellers WR. Nat Commun. 2022 May 5;13(1):2469. doi: 10.1038/s41467-022-30196-9. 10.1038/s41467-022-30196-9 PubMed 35513429
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