pWRS_1001
(Plasmid
#192205)
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Purpose(Empty Backbone) lentiviral expression of SpCas9 and two pol III promoters for dual expression of sgRNAs
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 192205 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepWRS1001
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Vector typeLentiviral, RNAi, CRISPR
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Cloning Information
- Cloning method Restriction Enzyme
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pWRS_1001 was a gift from William Sellers (Addgene plasmid # 192205 ; http://n2t.net/addgene:192205 ; RRID:Addgene_192205) -
For your References section:
Comparative optimization of combinatorial CRISPR screens. Li R, Klingbeil O, Monducci D, Young MJ, Rodriguez DJ, Bayyat Z, Dempster JM, Kesar D, Yang X, Zamanighomi M, Vakoc CR, Ito T, Sellers WR. Nat Commun. 2022 May 5;13(1):2469. doi: 10.1038/s41467-022-30196-9. 10.1038/s41467-022-30196-9 PubMed 35513429
Map uploaded by the depositor.
