Clone 16 - 2 HH06 pFUSE-IgG1-Knob-mutation knob-clone6
(Plasmid
#192184)
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PurposeClone 16 (HH06) pFUSE-IgG1-Knob-mutation knob-clone6
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Depositing Lab
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Sequence Information
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Sequences (1) — Accept Affinity Reagent Sequence Policy
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Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 192184 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepFuse
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Bleocin (Zeocin), 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameClone 6 heavy chain (HH06)
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SpeciesSynthetic
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MutationNA
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Clone 16 - 2 HH06 pFUSE-IgG1-Knob-mutation knob-clone6 was a gift from Sidi Chen (Addgene plasmid # 192184 ; http://n2t.net/addgene:192184 ; RRID:Addgene_192184) -
For your References section:
Monospecific and bispecific monoclonal SARS-CoV-2 neutralizing antibodies that maintain potency against B.1.617. Peng L, Hu Y, Mankowski MC, Ren P, Chen RE, Wei J, Zhao M, Li T, Tripler T, Ye L, Chow RD, Fang Z, Wu C, Dong MB, Cook M, Wang G, Clark P, Nelson B, Klein D, Sutton R, Diamond MS, Wilen CB, Xiong Y, Chen S. Nat Commun. 2022 Mar 28;13(1):1638. doi: 10.1038/s41467-022-29288-3. 10.1038/s41467-022-29288-3 PubMed 35347138