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Addgene

pVPSBA01-SARS-CoV2-nsp1-truncated
(Plasmid #192172)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 192172 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pVPSB
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    express SARS2-NSP1-trunc
  • Species
    SARS-CoV-2
  • Mutation
    NA
  • Entrez Gene
    ORF1ab (a.k.a. GU280_gp01)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pVPSBA01-SARS-CoV2-nsp1-truncated was a gift from Sidi Chen (Addgene plasmid # 192172 ; http://n2t.net/addgene:192172 ; RRID:Addgene_192172)

  • For your References section:

    Nonstructural Protein 1 of SARS-CoV-2 Is a Potent Pathogenicity Factor Redirecting Host Protein Synthesis Machinery toward Viral RNA. Yuan S, Peng L, Park JJ, Hu Y, Devarkar SC, Dong MB, Shen Q, Wu S, Chen S, Lomakin IB, Xiong Y. Mol Cell. 2020 Dec 17;80(6):1055-1066.e6. doi: 10.1016/j.molcel.2020.10.034. Epub 2020 Oct 29. 10.1016/j.molcel.2020.10.034 PubMed 33188728
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