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Addgene

pGW011b Dual AAV vector pAAV-EFS-dCAS9-spA
(Plasmid #192160)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 192160 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Dead Cas9
  • Species
    Synthetic
  • Mutation
    NA

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGW011b Dual AAV vector pAAV-EFS-dCAS9-spA was a gift from Sidi Chen (Addgene plasmid # 192160 ; http://n2t.net/addgene:192160 ; RRID:Addgene_192160)

  • For your References section:

    Multiplexed activation of endogenous genes by CRISPRa elicits potent antitumor immunity. Wang G, Chow RD, Bai Z, Zhu L, Errami Y, Dai X, Dong MB, Ye L, Zhang X, Renauer PA, Park JJ, Shen L, Ye H, Fuchs CS, Chen S. Nat Immunol. 2019 Nov;20(11):1494-1505. doi: 10.1038/s41590-019-0500-4. Epub 2019 Oct 14. 10.1038/s41590-019-0500-4 PubMed 31611701
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