pGW011b Dual AAV vector pAAV-EFS-dCAS9-spA
(Plasmid
#192160)
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PurposeDual AAV vector AAV-dCas9
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 192160 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
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SerotypeSelect serotype for details See details about
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PricingSelect serotype and quantity $ USD for preparation of µL virus + $30 USD for plasmid.
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Backbone
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Vector backbonepAAV
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameDead Cas9
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SpeciesSynthetic
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MutationNA
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGW011b Dual AAV vector pAAV-EFS-dCAS9-spA was a gift from Sidi Chen (Addgene plasmid # 192160 ; http://n2t.net/addgene:192160 ; RRID:Addgene_192160) -
For your References section:
Multiplexed activation of endogenous genes by CRISPRa elicits potent antitumor immunity. Wang G, Chow RD, Bai Z, Zhu L, Errami Y, Dai X, Dong MB, Ye L, Zhang X, Renauer PA, Park JJ, Shen L, Ye H, Fuchs CS, Chen S. Nat Immunol. 2019 Nov;20(11):1494-1505. doi: 10.1038/s41590-019-0500-4. Epub 2019 Oct 14. 10.1038/s41590-019-0500-4 PubMed 31611701