U6-Nme2_tracr
(Plasmid
#192146)
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PurposeExpresses Nme2-sgRNA cloned with BspQI
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 192146 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneAAV2 ITR vector
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Vector typeMammalian Expression, AAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNme2 sgRNA scaffold
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SpeciesSynthetic
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
U6-Nme2_tracr was a gift from Yongming Wang (Addgene plasmid # 192146 ; http://n2t.net/addgene:192146 ; RRID:Addgene_192146) -
For your References section:
Closely related type II-C Cas9 orthologs recognize diverse PAMs. Wei J, Hou L, Liu J, Wang Z, Gao S, Qi T, Gao S, Sun S, Wang Y. Elife. 2022 Aug 12;11. pii: 77825. doi: 10.7554/eLife.77825. 10.7554/eLife.77825 PubMed 35959889