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Addgene

Nsp2-SmuCas9_AAV
(Plasmid #192144)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 192144 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    AAV2 ITR vector
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Nsp2-SmuCas9
  • Species
    Synthetic

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Nsp2-SmuCas9_AAV was a gift from Yongming Wang (Addgene plasmid # 192144 ; http://n2t.net/addgene:192144 ; RRID:Addgene_192144)

  • For your References section:

    Closely related type II-C Cas9 orthologs recognize diverse PAMs. Wei J, Hou L, Liu J, Wang Z, Gao S, Qi T, Gao S, Sun S, Wang Y. Elife. 2022 Aug 12;11. pii: 77825. doi: 10.7554/eLife.77825. 10.7554/eLife.77825 PubMed 35959889
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