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Addgene

Sha2Cas9-HF
(Plasmid #192142)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 192142 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    AAV2 ITR vector
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Sha2Cas9-HF
  • Species
    Synthetic
  • Mutation
    Sha2Cas9-HF (R247A and N415A)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Sha2Cas9-HF was a gift from Yongming Wang (Addgene plasmid # 192142 ; http://n2t.net/addgene:192142 ; RRID:Addgene_192142)

  • For your References section:

    Identification of SaCas9 orthologs containing a conserved serine residue that determines simple NNGG PAM recognition. Wang S, Tao C, Mao H, Hou L, Wang Y, Qi T, Yang Y, Ong SG, Hu S, Chai R, Wang Y. PLoS Biol. 2022 Nov 30;20(11):e3001897. doi: 10.1371/journal.pbio.3001897. eCollection 2022 Nov. 10.1371/journal.pbio.3001897 PubMed 36449487
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