Sha3Cas9
(Plasmid
#192136)
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PurposeExpresses Sha3Cas9, and cloning backbone for sgRNA
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 192136 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneAAV2 ITR vector
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Vector typeMammalian Expression, AAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSha3Cas9
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SpeciesSynthetic
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Sha3Cas9 was a gift from Yongming Wang (Addgene plasmid # 192136 ; http://n2t.net/addgene:192136 ; RRID:Addgene_192136) -
For your References section:
Compact SchCas9 Recognizes the Simple NNGR PAM. Wang S, Mao H, Hou L, Hu Z, Wang Y, Qi T, Tao C, Yang Y, Zhang C, Li M, Liu H, Hu S, Chai R, Wang Y. Adv Sci (Weinh). 2022 Feb;9(4):e2104789. doi: 10.1002/advs.202104789. Epub 2021 Dec 6. 10.1002/advs.202104789 PubMed 34874112