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Addgene

Sha3Cas9
(Plasmid #192136)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 192136 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    AAV2 ITR vector
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Sha3Cas9
  • Species
    Synthetic

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Sha3Cas9 was a gift from Yongming Wang (Addgene plasmid # 192136 ; http://n2t.net/addgene:192136 ; RRID:Addgene_192136)
  • For your References section:

    Compact SchCas9 Recognizes the Simple NNGR PAM. Wang S, Mao H, Hou L, Hu Z, Wang Y, Qi T, Tao C, Yang Y, Zhang C, Li M, Liu H, Hu S, Chai R, Wang Y. Adv Sci (Weinh). 2022 Feb;9(4):e2104789. doi: 10.1002/advs.202104789. Epub 2021 Dec 6. 10.1002/advs.202104789 PubMed 34874112