SchBE4max
(Plasmid
#192135)
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PurposeExpresses AncBE4max and nSchCas9
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 192135 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneExpression vector pKK44
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAncBE4max, nSchCas9
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SpeciesSynthetic
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MutationSchCas9 (D9A)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
SchBE4max was a gift from Yongming Wang (Addgene plasmid # 192135 ; http://n2t.net/addgene:192135 ; RRID:Addgene_192135) -
For your References section:
Compact SchCas9 Recognizes the Simple NNGR PAM. Wang S, Mao H, Hou L, Hu Z, Wang Y, Qi T, Tao C, Yang Y, Zhang C, Li M, Liu H, Hu S, Chai R, Wang Y. Adv Sci (Weinh). 2022 Feb;9(4):e2104789. doi: 10.1002/advs.202104789. Epub 2021 Dec 6. 10.1002/advs.202104789 PubMed 34874112