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Addgene

pSK-mU6-Hsp1-sgRNA-SV40-puro
(Plasmid #192129)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 192129 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC57 vector
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Hsp1-SgRNA scaffold
  • Species
    Synthetic

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSK-mU6-Hsp1-sgRNA-SV40-puro was a gift from Yongming Wang (Addgene plasmid # 192129 ; http://n2t.net/addgene:192129 ; RRID:Addgene_192129)

  • For your References section:

    Genome editing with natural and engineered CjCas9 orthologs. Gao S, Wang Y, Qi T, Wei J, Hu Z, Liu J, Sun S, Liu H, Wang Y. Mol Ther. 2023 Apr 5;31(4):1177-1187. doi: 10.1016/j.ymthe.2023.01.029. Epub 2023 Feb 1. 10.1016/j.ymthe.2023.01.029 PubMed 36733251
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