pVRC8400-SARS-CoV-2-NTD-BA.4/5-AVI
(Plasmid
#192113)
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PurposeExpresses SARS-CoV-2 NTD protein from the BA.4 and BA.5 variants with single chain Fc, HRV3C protease cleavage site, and AVI tag
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 192113 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepVRC8400
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameSARS-CoV-2-NTD-BA.4/5
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MutationT19I, L24S, P25-, P26-, A27-, H69-, V70-, G142D, V213G
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Entrez GeneS (a.k.a. GU280_gp02, spike glycoprotein)
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Tags
/ Fusion Proteins
- scFc (N terminal on insert)
- HRV3C (N terminal on insert)
- AVI (C terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pVRC8400-SARS-CoV-2-NTD-BA.4/5-AVI was a gift from Peter Kwong (Addgene plasmid # 192113 ; http://n2t.net/addgene:192113 ; RRID:Addgene_192113)