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pLenti4/V5-DEST-LASSIE
(Plasmid #192105)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 192105 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLenti4/V5-DEST™ Gateway™ vector
  • Backbone manufacturer
    Thermo Scientific
  • Backbone size w/o insert (bp) 7000
  • Total vector size (bp) 7400
  • Vector type
    Lentiviral
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    LASSIE
  • Alt name
    linc00520
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    393
  • Promoter CMV

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti4/V5-DEST-LASSIE was a gift from Reinier Boon (Addgene plasmid # 192105 ; http://n2t.net/addgene:192105 ; RRID:Addgene_192105)

  • For your References section:

    Long non-coding RNA LASSIE regulates shear stress sensing and endothelial barrier function. Stanicek L, Lozano-Vidal N, Bink DI, Hooglugt A, Yao W, Wittig I, van Rijssel J, van Buul JD, van Bergen A, Klems A, Ramms AS, Le Noble F, Hofmann P, Szulcek R, Wang S, Offermanns S, Ercanoglu MS, Kwon HB, Stainier D, Huveneers S, Kurian L, Dimmeler S, Boon RA. Commun Biol. 2020 May 26;3(1):265. doi: 10.1038/s42003-020-0987-0. 10.1038/s42003-020-0987-0 PubMed 32457386
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