V5-K202R-short-UBASH3A
(Plasmid
#192084)
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PurposeMammalian expression of human K202R short-form UBASH3A with V5 tag
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 192084 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1/nV5-DEST
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameUBASH3A
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SpeciesH. sapiens (human)
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MutationK202R
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Entrez GeneUBASH3A (a.k.a. TULA, CLIP4, STS-2)
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Tag
/ Fusion Protein
- V5 (N terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
V5-K202R-short-UBASH3A was a gift from Patrick Concannon (Addgene plasmid # 192084 ; http://n2t.net/addgene:192084 ; RRID:Addgene_192084) -
For your References section:
UBASH3A Regulates the Synthesis and Dynamics of TCR-CD3 Complexes. Ge Y, Paisie TK, Chen S, Concannon P. J Immunol. 2019 Dec 1;203(11):2827-2836. doi: 10.4049/jimmunol.1801338. Epub 2019 Oct 28. 10.4049/jimmunol.1801338 PubMed 31659016