pMis1_1B
(Plasmid #192079)

• Purpose: A pBBR-1 based vector with IncP group plasmid compatibility for expression in Methylorubrum extorquens using rhamnose dependent induction
• Depositing Lab: Markus Buchhaupt
• Publication: Poschel et al Microbiologyopen. 2022 Oct;11(5):e1325. doi: 10.1002/mbo3.1325.

Backbone

• Vector backbone: pJeM1
• Backbone manufacturer: Jeske M, Altenbuchner J
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: KanR
• Alt name: aminoglycoside phosphotransferase from Tn5
• Species: transposon Tn5
• Insert Size (bp): 795

Gene/Insert 2

• Gene/Insert name: rhaR
• Alt name: DNA-binding transcriptional activator RhaR from Escherichia coli
• Species: Escherichia coli
• Insert Size (bp): 939
• GenBank ID: 948396

Gene/Insert 3

• Gene/Insert name: rhaS
• Alt name: DNA-binding transcriptional activator RhaS from Escherichia coli, Gene ID: 948397, positive regulator of the rhaB promoter
• Insert Size (bp): 837
• GenBank ID: 948397

Gene/Insert 4

• Gene/Insert name: pBBR1 rep
• Alt name: replication protein for the broad-host-range plasmid pBBR1 from Bordetella bronchiseptica
• Species: Bordetella bronchiseptica
• Insert Size (bp): 663

Resource Information

• Supplemental Documents:
  • pMis1_1B.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry