pQE-meGFP-DLC1
(Plasmid
#192048)
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PurposePlasmid expresses DLC1 binding peptide to Talin R8 domain in chimera with meGFP
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 192048 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonemeGFP-DLC1
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameDLC1
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SpeciesH. sapiens (human)
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Entrez GeneDLC1 (a.k.a. ARHGAP7, HP, STARD12, p122-RhoGAP)
Cloning Information
- Cloning method TOPO Cloning
- 5′ sequencing primer unknown (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pQE-meGFP-DLC1 was a gift from Ionel Popa (Addgene plasmid # 192048 ; http://n2t.net/addgene:192048 ; RRID:Addgene_192048) -
For your References section:
Mechanical regulation of talin through binding and history-dependent unfolding. Dahal N, Sharma S, Phan B, Eis A, Popa I. Sci Adv. 2022 Jul 15;8(28):eabl7719. doi: 10.1126/sciadv.abl7719. Epub 2022 Jul 15. 10.1126/sciadv.abl7719 PubMed 35857491