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Addgene

pFN-HaloTag-TalinR7R8-SpyTag
(Plasmid #192046)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 192046 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Talin R7R8
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TLN1
  • Species
    H. sapiens (human)
  • Entrez Gene
    TLN1 (a.k.a. ILWEQ, TLN, talin-1)
  • Tags / Fusion Proteins
    • HaloTag (N terminal on insert)
    • SpyTag (C terminal on insert)

Cloning Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFN-HaloTag-TalinR7R8-SpyTag was a gift from Ionel Popa (Addgene plasmid # 192046 ; http://n2t.net/addgene:192046 ; RRID:Addgene_192046)

  • For your References section:

    Mechanical regulation of talin through binding and history-dependent unfolding. Dahal N, Sharma S, Phan B, Eis A, Popa I. Sci Adv. 2022 Jul 15;8(28):eabl7719. doi: 10.1126/sciadv.abl7719. Epub 2022 Jul 15. 10.1126/sciadv.abl7719 PubMed 35857491
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