pFN-HaloTag-TalinR8-SpyTag
(Plasmid
#192045)
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PurposePlasmid expresses human talin R8 domain flanked between a HaloTag and a SpyTag
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 192045 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneTalin R8
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTLN1
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SpeciesH. sapiens (human)
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Entrez GeneTLN1 (a.k.a. ILWEQ, TLN, talin-1)
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Tags
/ Fusion Proteins
- HaloTag (N terminal on insert)
- SpyTag (C terminal on insert)
Cloning Information
- Cloning method TOPO Cloning
- 5′ sequencing primer unknown (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFN-HaloTag-TalinR8-SpyTag was a gift from Ionel Popa (Addgene plasmid # 192045 ; http://n2t.net/addgene:192045 ; RRID:Addgene_192045) -
For your References section:
Mechanical regulation of talin through binding and history-dependent unfolding. Dahal N, Sharma S, Phan B, Eis A, Popa I. Sci Adv. 2022 Jul 15;8(28):eabl7719. doi: 10.1126/sciadv.abl7719. Epub 2022 Jul 15. 10.1126/sciadv.abl7719 PubMed 35857491
