p3xFLAG-CMV-14-Mfn1-T564A
(Plasmid
#191978)
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PurposeTo produce mutants mimicking dephosphorylated Mfn1
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 191978 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonep3xFLAG-CMV-14
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameMfn1 T564A
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SpeciesM. musculus (mouse)
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MutationT564A
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Entrez GeneMfn1 (a.k.a. 2310002F04Rik, 6330416C07Rik, D3Ertd265e, HR2, mKIAA4032)
Cloning Information
- Cloning method Unknown
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p3xFLAG-CMV-14-Mfn1-T564A was a gift from Luca Scorrano (Addgene plasmid # 191978 ; http://n2t.net/addgene:191978 ; RRID:Addgene_191978) -
For your References section:
Extracellular regulated kinase phosphorylates mitofusin 1 to control mitochondrial morphology and apoptosis. Pyakurel A, Savoia C, Hess D, Scorrano L. Mol Cell. 2015 Apr 16;58(2):244-54. doi: 10.1016/j.molcel.2015.02.021. Epub 2015 Mar 19. 10.1016/j.molcel.2015.02.021 PubMed 25801171