pcDNA3.1/Zeo-CnA-H151Q
(Plasmid
#191967)
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PurposeExpression of dominant negative CnA H151Q mutant protein (catalytically inactive).
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 191967 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1/Zeo
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCalcineurin subunit alpha H151Q
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SpeciesH. sapiens (human)
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MutationH151Q
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Entrez GenePPP3CA (a.k.a. ACCIID, CALN, CALNA, CALNA1, CCN1, CNA1, DEE91, IECEE, IECEE1, PPP2B)
Cloning Information
- Cloning method Unknown
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.1/Zeo-CnA-H151Q was a gift from Luca Scorrano (Addgene plasmid # 191967 ; http://n2t.net/addgene:191967 ; RRID:Addgene_191967) -
For your References section:
Dephosphorylation by calcineurin regulates translocation of Drp1 to mitochondria. Cereghetti GM, Stangherlin A, Martins de Brito O, Chang CR, Blackstone C, Bernardi P, Scorrano L. Proc Natl Acad Sci U S A. 2008 Oct 14;105(41):15803-8. doi: 10.1073/pnas.0808249105. Epub 2008 Oct 6. 10.1073/pnas.0808249105 PubMed 18838687