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pBS Matrilin-4 (clone 1_35)
(Plasmid #19192)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 19192 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBluescript
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Matrilin 4 isoform 1 precursor
  • Alt name
    Matrilin 4
  • Species
    G. gallus (chicken)
  • GenBank ID
    FG356181
  • Entrez Gene
    MATN4

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (unknown if destroyed)
  • 3′ cloning site EcoRI (unknown if destroyed)
  • 5′ sequencing primer T3
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Probe 1_35, expression pattern is somewhere in between ABI3BP and CRABP1, expression is very prominent in the tendons and very different from 2_49 (another matrilin 4 isoform 1 precursor).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBS Matrilin-4 (clone 1_35) was a gift from Cliff Tabin (Addgene plasmid # 19192 ; http://n2t.net/addgene:19192 ; RRID:Addgene_19192)

  • For your References section:

    Identification of unique molecular subdomains in the perichondrium and periosteum and their role in regulating gene expression in the underlying chondrocytes. Bandyopadhyay A, Kubilus JK, Crochiere ML, Linsenmayer TF, Tabin CJ. Dev Biol. 2008 Sep 1;321(1):162-74. doi: 10.1016/j.ydbio.2008.06.012. Epub 2008 Jun 16. 10.1016/j.ydbio.2008.06.012 PubMed 18602913
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